2020, Volume 14, Issue 2
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RekordAssessing knowledge of physical activity benefits in diabetic patients(Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, 2020-05-27) Junger, Jan ; Dračková, Dana ; Buková, Alena ; Junger, Andrea ; Kuchelová, ZuzanaBackground. The aim of this work was to examine the awareness of patients with diabetes mellitus (DM) on the importance of physical activity (PA) in the prevention and treatment of their disease. Material and methods. The survey was completed by 178 respondents (47.3% women, 52.7% men), of whom 69.1% were dependent on exogenous insulin. Data was collected in April 2019 via a questionnaire given to patients at the Department of Diabetology and Internal Medicine at the Louis Pasteur University Hospital in Košice, Slovakia. Results. More than half of the respondents admitted having insufficient information about their health condition, and a similar proportion felt lack of knowledge regarding exercise for their illness. As many as 69.1% of the patients reported inadequate help by their health care providers with regard to the do’s and dont’s of their disease, and 41.9% of respondents reported having no knowledgeable health care provider with whom to share their concerns. Conclusions. Based on the results obtained, it is concluded that a substantial number of DM patients do not have adequate information about their health condition and how to improve it. On the other hand, nearly two-thirds of participants recognize that regular physical activity (PA) is an option to achieve positive changes.
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RekordCurrent methods used to identify and genotype spirochaetes Borreliella burgdorferi(Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, 2020-04-22) Weiner, Marcin ; Teodorowicz, PatrycjaLyme disease, as one of tick-borne diseases, has been a current epidemiological problem in Poland and in the world for several years. The proportion of borreliosis infections caused by B. burgdorferi spirochaetes is increasing. Difficulties diagnosing this disease with conventional methods have led to growing molecular biology research aimed at developing improved diagnostic tools. Molecular biology methods include all techniques that allow the detection and analysis of nucleic acids. Among them there are methods based on PCR reaction, molecular typing methods (MLST), new generation sequencing (NGS). The current development of this field gives great research opportunities. With regard to B. burgdorferi spirochaete, molecular biology is used to: confirm Lyme borreliosis, identify and type Borreliella spirochaetes, detect them in tick vectors or intermediate hosts, as well as to identify co-infections between different Borreliella species and other tick-borne pathogens. They are meant to significantly improve diagnostic analyzes. This paper reviews the current methods used for the detection and identification of B. burgdorferi. Molecular mechanisms for the survival of spirochaetes in the host, infection vectors and clinical picture of Lyme disease were also discussed.
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RekordThe effect of sodium selenite treatment in an experimental model of acute glyphosphate poisoning(Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, 2020-05-27) Oliynyk, OleksandrBackground. The aim of this study was to investigate the possibility of using sodium selenite as a treatment for acute glyphosate poisoning using the activity of the lipid peroxidation and antioxidant defence systems as a readout for efficacy. Material and methods. Experimental glyphosate poisoning and subsequent treatment using sodium selenite was performed in albino rats (105). Glyphosate was given in doses of 50, 100 and 130 mg/kg, and sodium selenite was administered at a dose of 2 μg/kg. The blood concentrations of lipid peroxidation markers including conjugates of diene andtrienoic and malondialdehyde were determined. The endogenous glutathione (reduced form) level and activities of catalase, superoxide dismutase and glutathione peroxidase in the serum were measured. Results. Glyphosate poisoning has been found to result in a significant increase in lipid peroxidation activity. For example, malonic dialdehyde demonstrates a 2.35 times increase at a glyphosate dose of 130 mg/kg. At the experimental glyphosate poisoning dose of 100 mg/kg the measurements of superoxide dismutase and glutathione peroxidase have been found to decrease 1.58 and 2.21 times, respectively. At a dose of 130 mg/kg, those values decreased 2.51 and 4.76 times, respectively, compared to untreated controls. Conclusions. The use of sodium selenite at a dose of 2 μg/kg after poisoning of white rats with glyphosate (at doses of 50, 100 and 130 mg/kg) normalizes the lipid peroxidation and antioxidant defence activities of the body.
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RekordThe efficiency of high-frequency endovenous welding for the surgical treatment of acute ascending thrombophlebitis of the great saphenous vein(Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, 2020-04-22) Glagolieva, Anastasiia ; Gerashchenko, Roman ; Kurmanskyi, Andrii ; Gorbovets, Vladyslav ; Khodos, Valentyna ; Savolyuk, SergiiBackground. Acute ascending thrombophlebitis of the great saphenous vein (GSV) is an adverse complication of varicose veins of the lower extremities. Conventional surgical treatment comprises traumatic removal of thrombotic vein trunk by means of a Babcock probe. Endovascular welding is a promising, minimally invasive treatment for varicose veins, which may also be applied for hemodynamic correction of impaired venous blood flow of the lower extremities in urgent conditions. Material and methods. Thirty-six patients diagnosed with acute ascending thrombophlebitis type III and IV, stage C2-C6 (CEAP) were treated using an EK300M electric welding device (LLC Svarmed, Ukraine) for high-frequency electric welding of living tissues guided by endovenous probes. Changes in the treated venous trunks were analyzed by ultrasound, and quality of life before and after surgical treatment was evaluated using a chronic insufficiency venous international questionnaire (CIVIQ). Results. Twelve months after high-frequency endovenous electric welding 33 patients (91.41%) had complete occlusion and fibrous transformation in areas of the thrombus segments of the GSV. Two patients (5.54%) had hemodynamically insignificant partial recanalization of separate vein segments, and one patient (2.77%) had complete recanalization of the GSV trunk. Conclusions. Endoscopic welding is an effective, minimally traumatic alternative method for the surgical treatment of acute ascending thrombophlebitis III and VI type of the GSV.
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RekordOccurrence of Candida spp. in healthy oral microbiota(Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, 2020-04-21) Kimsa, Łukasz ; Tokarska-Rodak, MałgorzataBackground. Candida is a strain of fungi that is found on the surface of healthy mucous membranes of the oral cavity. Yet, Candida may also be considered opportunistic microorganisms. The pathogenicity of Candida spp. depends on strain-specific characteristics including, invasiveness, adhesion, and production of biofilm abilities as well as the production of enzymes allowing the colonization of tissue. The aim of the paper was to evaluate the frequency of Candida spp. occurrence in the oral microbiota of healthy adults, and its relation with risk factors and colonization. Material and methods. This study consisted of 72 healthy participants (from the Lubelskie Voivodeship between 19 and 73 years of age). Medical history was collected to determine potential relationships to risk factors affecting oral microbiota (past oral cavity diseases, chronic diseases, and smoking). The participants who were qualified had no oral cavity infections during the study. The collected clinical samples (oral cavity swabs) were disseminated on the chromogenic base for isolation and differentiation of Candida spp. Results. The frequency of Candida spp. occurrence was at the level of 63.8%. C. albicans was the most frequently identified species (56.5%). C. glabrata (45.6%) and C. tropicalis (23.9%) were identified less often. In 21.7% of swabs, two species of Candida were identified and in 2.2% of swabs three species identified. The most common colonization risk factor was associated with smoking (32.6%). Conclusions. Yeasts of Candida species are part of the healthy microflora of the oral cavity in people of different ages and may occur as single species or coexist with other species. The coexistence of chronic diseases, propensity for oral cavity infections and smoking significantly influence the Candida colonization. This may result in uture clinical consequences, for example in cases of immunodepression.
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RekordOccurrence of Legionella spp. in selected public facilities in Poland in 2009-2013 and 2014-2016: a comparative study(Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, 2020-05-11) Karczewski, Jan KazimierzBackground. This paper evaluated Legionella spp. water system contamination in the public facilities. Material and methods. Samples of warm water from the inner water supply system of 41 facilities in years 2009-2013 and 46 facilities in years 2014-2016 were collected for analyses. The facilities included school dormitories, boarding houses, school-education centers, halls of residence, nursery schools, kindergartens, and correctional institutions. Results. The results of research carried out on tap water in public facilities in 2014-2016 showed that in comparison to 2009-2013 the percentage of facilities from group I increased. In group III facilities a decreasing tendency was observed in comparison to research carried out in 2009-2013. Conclusions. The results indicate that it cannot be explicitly ascertained that the eradication methods of L. pneumophila from tap water systems of the analyzed facilities within the studied period were sufficient. Epidemiological analyses of this type should be successively and systematically continued and based of the largest possible number of facilities.
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RekordPhysical activity of physiotherapy students and the role of device-based monitoring in their future clinical practice: a cohort study(Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, 2020-05-10) Michalčíková, Tamara ; Vorlíček, Michal ; Pechová, Jana ; Jakubec, Lukáš ; Frömel, Karel ; Neumannová, KateřinaBackground. Although physiotherapy students are expected to promote and prescribe exercise, their own physical activity (PA) levels have not been fully researched using wearable devices to provide objective measurements of PA. This study aimed (1) to determine their PA level; and (2) to verify the use of wearables for physiotherapy students’ own practical benefits. Material and methods. Students (n=257) wore a pedometer (Yamax Digi-Walker SW-700) and wrist-based activity tracker (Garmin Vívofit 1) for seven consecutive days. Prior to monitoring, they completed the long form of the International Physical Activity Questionnaire (IPAQ-LF). A further evaluation was completed, relating to benefits and shortcomings of PA monitoring in their own practice. Results. A significant difference emerged between PA levels recorded with activity trackers on weekdays and weekend days, but only when gender and years of study were not taken into consideration. Specifically, 61.09% of participants were active or highly active on weekdays and none were sedentary. On the weekend, however, 47.47% were active or highly active and 11.67% were sedentary. Most (~79%) subjects reported clear benefits in PA monitoring. Conclusions.The student cohort were clearly active during the week, but almost half were insufficiently active in the weekend and thus, greater focus on the promotion of PA in physiotherapists-in-training might be necessary. The self-monitoring of PA under ambulatory conditions (using two wearable devices), combined with self-evaluation of PA, were study strengths.
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RekordPhysioprophylaxis in physiotherapy and health promotion(Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, 2020-05-27) Kubińska, Zofia ; Zaworski, Kamil ; Shpakou, AndreiAccording to the authors, physioprophylaxis is a preventive measure against developmental and lifestyle diseases, the aging process and disabilities through: ergonomic performance of daily and professional activities; systematic physical exercise; behaviors that strengthen pro-health factors; and which is oriented towards risk prevention. In the health care system, it is a new and effective prophylactic alternative for the prevention of ever-increasing health risks. As a health need and medical service, physioprophylaxis has officially become present in the law, standards of education and in the professional practice of most physiotherapists. On the other hand, the promotion of the health and well-being of people and the general public that emphasizes the importance of physical activity and exercise is one of a physiotherapist’s professional objectives. The aim of this work is to present an exemplary description of physioprophylaxis integrated with health promotion. The authors resolved to show the types, means and methods of its impact on health education, risk prevention and health policy.
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RekordPrevalence of the factor V Leiden mutation in patients susceptible to venous thromboembolism(Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, 2020-04-22) Mitrus, Joanna Małgorzata ; Wierszyło, ElżbietaBackground. A tendency to venous thromboembolism is otherwise called hypercoagulability or thrombophilia. This disease can be acquired or have a genetic background, and may lead to pulmonary embolism. The basis for analysis and selection of treatment is genetic diagnosis, which detects the G1691A mutation in the factor V gene (factor V Leiden) – the best known congenital thrombophilia marker. Material and methods. The study was carried out in the years 2015-2017 on samples taken from patients (462 men and 1284 women) with a tendency to venous thromboembolism. Real-Time PCR was used to detect G1691A mutation in factor V gene. The analyses were performed in the Hematology Laboratory of the Center of Laboratory Medicine at the Medical University of Gdańsk. Results. Significant differences in the frequency of Leiden mutation were shown. This mutation predominated in men (25%), while in women G1691A mutation was detected with a 15% frequency (p=0.04). All possible genotypes were found among the subjects and the percentage of heterozygotes and homozygotes in both genders was similar. Conclusions. Congenital thrombophilia associated with G1691A mutation of factor V Leiden gene was found to be more common in men than in women. All possible genotypes were determined in the pool of test subjects. The mutation was most frequently detected in patients between 30 and 40 years of age, and rarely after 70 years of age.
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RekordThe effectiveness of deep transverse friction massage for the treatment of medial meniscotibial ligament injury in a female football player: a case study(Państwowa Szkoła Wyższa im. Papieża Jana Pawła II w Białej Podlaskiej, 2020-05-10) Zaworski, KamilBackground. The injury to medial meniscotibial ligament (MTL) is a cause of pain in the medial articulatio genus which is rarely discussed in the literature. The paper examines the effectiveness of deep transverse friction massage (DTFM) in the treatment of injury to the medial meniscus-tibial ligament in a female football player. Material and methods. A case study concerned a patient (22 years old) who is a football player on the position of striker. She has been complaining about the pain in the right, medial articulatio genus for five days. Deep transverse friction massage (DTFM) was applied in the treatment. A total of 9 procedures was conducted (3 times a week) throughout three weeks. The first DTFM session lasted 2 minutes and the time was systematically extended during further sessions until it reached 15 minutes. Visual Analogue Scale (VAS), modified Laitinen Pain Questionnaire and countermovement jump test (CMJ) on the dynamometric platform were applied to evaluate the effectiveness of the treatment. Results. Having completed the treatment, a decrease of pain measured by the means of VAS and Laitinen Pain Questionnaire was reported. There was also improvement in limb symmetry index in all phases of the test carried out on the dynamometric platform. Conclusions. The results demonstrate that DTFM may be an effective method of treatment for MTL injuries. Conducting further research on a bigger study group is necessary.